Is it experimentally feasible to replicate immune tolerance in a basic in vitro transfusion model?
Hi everyone, I’m currently a student exploring transfusion immunology. I’ve been fascinated by maternal-fetal immune tolerance mechanisms, and I’m wondering if we can translate these into a basic in vitro transfusion model to mitigate alloimmunization. I’m trying to design an in vitro assay to test this, but I’m getting conflicting ideas. Some sources suggest that selective antigen-filtering or Treg modulation could work, but others argue that the complexity of the immune response makes a small-scale model virtually impossible to validate. Since there isn't much clear evidence for a small-scale setup, I’m stuck. For those with experience, do you think it's experimentally feasible to model these pathways in vitro, or are the clinical variables just too complex to replicate accurately? I’d love to hear different perspectives on whether this approach has any real potential or if it's just a theoretical dead end. I'm using a translator to write this, so please bear with me. Any insights or arguments for either side would be incredibly valuable!