Naphta in my country is VERY expensive, For back salts can I use Lantern oil that’s non polar and doesn’t have a lot of chemicals inside? Also it doesn’t remain in the mixture and separates fast (Im not planning to extract with it i want to remove oils, waxes and lipids.)
Samples were prepared by soaking plant material in a mixture of aqueous NH₃ and MTBE to isolate relatively nonpolar compounds.
Extracts were applied to unmodified 60 Å non-fluorescent silica plates and developed using a mobile phase consisting of IPA and aqueous NH₃.
With careful specimen selection, some plants produced remarkably clean chromatographic profiles, approaching near single-constituent extracts in which one major alkaloid dominates while others are present only in trace amounts.
Gramine and β-carboline and 5-MeO-DMT content can be minimized through by careful selection.
These samples were preselected for clean profiles and sourced within the r/Phalaris community.
Image 1
Phalaris samples under 275 nm while still wet with mobile phase.
Non-methoxylated tryptamines exhibit royal blue fluorescence.
Image 2
Samples under 275 nm after drying.
Methoxylated tryptamines exhibit green fluorescence.
Image 3
Samples under 365 nm after drying.
β-carbolines exhibit green to cyan fluorescence.
Image 4
MHRB and Psychotria viridis dilution series under 275 nm while wet with mobile phase.
Image 5
MHRB and Psychotria viridis under 275 nm after drying.
Methoxylated tryptamines exhibit green fluorescence.
Image 6
MHRB and Psychotria viridis under 365 nm after drying.
β-carbolines exhibit green to cyan fluorescence.
Phalaris Acuatica.
Hola volvi de nuevo, estoy he ocupado con otros compuestos y plantas.
Pero bueno!
A trabajar! Mañana les muestro los resultados!
When I harvest I always have to check the base to see if it’s aquatica or not since in fields it’s easy to confuse a paradoxa or tall annual for Aquatica, Are there any other reliable features of Aquatica other than a red boulbous base?
I realized with the amount of grass I’m growing, I won’t be able to do a large scale extraction so I tried to come up with a potential safe anahuasca recipe.
First of, phalaris grass contains unpredictable levels of 5 meo dmt, which can be deadly when consumed with an MAOI. I read that 5 meo dmt is quite sensitive to high phs, so if a phalaris grass is boiled in water and vinegar, than that liquid with mixed with LARGE amount of lye, that liquid should contain only freebase forms of other tryptamines in the plant. So if someone shakes it up with small portions of olive oil, than mixes said olive oil with a Syrian rue or ayahuasca vine concentrate, they could mix the oil in a dish of their choice and make a sort of edible anahuasca.
I kinda feel like this is too good to be true though.
Hey, i have tested a few local P. arundinaceas.
I struggle with interpreting them. Is the second one having NMT?
Eluent is methanol + nh3.
Taking wet and dry images with 275nm works fine for me.
Following the transplanting of 750 F2 hybrids and 10 North African accessions, a preliminary bioassay was conducted on two high-yield DMT clones. These clones were selected from the hybrid plot based on Thin Layer Chromatography (TLC) analysis, specifically targeting non-methoxylated alkaloids.
- Material & Extraction: The plant material consisted of 8.5g of fresh leaves, which were subsequently dried to a final weight of 2.000g as determined by a 0.001g moisture analyzer. The extraction method involved boiling the dried leaf material twice in distilled water with citric acid, each for 30 minutes. The resulting solution was then basified with NaOH and extracted twice with chloroform. The chloroform pulls were combined and evaporated in a pre-weighed 50mL beaker. The crude extract yield was 18mg from 2g of dry leaf, representing a 0.9% crude yield (w/w dry weight). Based on dose-response, the crude extract was estimated to be approximately 50% DMT, resulting in a net DMT yield of approximately 0.3–0.4% of dry leaf.
Pic 5, 6 is the TLC analysis result of one of the two clones tested. Clone sample is lane 7. Lane 4 is MHRB. All lanes on the plate are 25mg dry weight samples soaked in 1ml chloroform + few drops of 25% ammonia. Notice the two largest blue fluorescing spots at the top (DMT RF) are the MHRB and the DMT clone (which based on it's DMT RF fluorecence intensity seems to be roughly 40% that of MHRB DMT spot, which is backed by the practical crude yeild extracted.
Pic 3 and 4 are the selected clones being transplanted for clonal propagation.
Pic 5 is the 8.5g fresh leaves extracted which gave the 18mg crude freebase.
Bioassay Results: The bioassay revealed that the onset, peak, duration, and subjective effects were strikingly similar to that of DMT extracted from MHRB.
To be absolutely sure i pulled out my Stash of MHRB extracted DMT freebase and vaporized some.. it only lead to increased intensity of the same effects i had from the hybrid clones extract. I wish i smoked the clone extract at higher dose all at once... The room was just starting to morph when i reached the peak of the last dose and had no more left to smoke.
I was smoking the. Rude freebase in incremental doses to explore it. Now that i am familiar with these selected clones benign qualities my next harvest i will go for breakthrough.
After years of dedicated selection and stabilization, we are opening an exchange for our advanced Phalaris aquatica breeding lines.
Year 1: Selection & Isolation
We screened multiple Phalaris species for phytochemical potential. P. aquatica emerged as the clear winner due to its clean profile, high biomass yield, genetic diversity, and vigorous adaptability to various climates. We isolated the top-performing wild specimens to begin our lineage.
Year 2: Genetic Crossing
We acquired 20 distinct wild accessions. After rigorous testing, we identified that only 10% met our potency requirements for DMT and 5-MeO-DMT. These elite individuals were crossed to create our F1 generation.
Year 3: Stabilization & Optimization
Current F1 testing shows a 90% high-yield success rate. Our most potent outliers are now 70% stronger than the highest-testing wild populations. Interestingly we have successfully selected against Gramine; metabolic precursors are now increasingly diverted toward the desired synthesis pathways.
Current Phenotype Groups
We are currently stabilizing three distinct chemotypes:
These seeds represent a significant leap in Phalaris breeding—cleaner and more potent than any commercially available or wild seed. I do not sell these seeds, they are too rare.
How to participate:
I am looking for serious collaborators who meet one of the following criteria:
Get in Touch:
If you want to join the project, need assistance setting up TLC for selection, or have wild P. aquatica growing in your region, please reach out via DM.
Apologies if I’m way off or if identification posts are not welcome here. I know you can’t really confirm with a picture or video but just wondering if it appears to be so. Thanks for any help, cheers.
Hey guys, i am glad i found this sub.
I was growing P. aquatica cv. AUSTRALIAN for some time now. Does it contain toxic tryptamines? Is it worth testing?
I want to harvest phalaris in may but I noticed some reeds are starting to dry (Some fully some have partially or fully dried flowers and leaves)
If so how’s its avg tryptamine concentration by dry weight?
I know MAOIS with 5-meo dmt is basically destroying your mind and potentially give you seratonin syndrome.