General Advice & RNA-seq help
Hi everyone,
I am currently a masters student and part of my research is using RNA-seq to look at DEGs in virus-infected vs virus-cured isolates of fungi. I don’t have any experience in bioinformatics (or genetics for that matter) and was looking for some tips/advice to help me learn how to get the hang of this stuff.
I’m also looking through NCBI SRA RNA-seq data , where I’ll be looking through a bunch of fungal isolates to see the diversity of viruses within them (probably a lot of them will be uncharacterized). Even just doing this has proven difficult, I guess you have to like parse through the data and “trim” reads and stuff like that and use “SRAtoolkit” , I’m just confused how people even know what to do/use in the first place.
Does anyone know of any free courses or programs that teaches the basics (any YouTube ppl? Or videos?)? I’ve only ever coded with R, and using the command line/my universities HPC cluster is proving difficult (I’ve looked at university resources and the HPC cluster website and they don’t have helpful tips for noobs like me). Yes , I am receiving some help from my PI, but as many of you know , they can be extremely busy. I feel like there is just a lot of assumed knowledge placed on me/grad students in general.
(Sorry if this isn’t a specific enough post, I can try to come up with more concrete questions if need be. Just looking for general advice/support :/ .)
Thank you in advance! I appreciate anyone who takes the time to respond :)