Tom Bateman: Bruce Wayne / Batman
Ralph Fiennes: Alfred Pennyworth
Ernie Hudson: Lucius Fox
Maya Hawke: Barbara Gordon / Batgirl
Asher Angel: Dick Grayson / Nightwing
Mason Thames: Jason Todd / Red Hood
Walker Scobell: Tim Drake / Robin
Gordon Cormier: Damien Wayne
Ethan Hawke: James Gordon
Donal Logue: Harvey Bullock
Michael Jai White: Aaron Cash
Stephanie Beatriz: Renee Montoya
Deborah Ann Woll: Vicki Vale
Heather Langenkamp: Dr Leslie Thompkins
Colin Farrell: Carmine Falcone
Willem Dafoe: The Joker
Margot Robbie: Harleen Quinzeel / Harley Quinn
Natalie Dreyfuss: Selina Kyle / Catwoman
Cory Michael Smith: Edward Nygma / The Riddler
Peter Dinklage: Oswald Cobblepot / The Penguin
Karen Gillan / Courtney Hope: Pamela Isley / Poison Ivy
John Noble: Jonathan Crane / Scarecrow
Sebastian Stan: Harvey Dent / Two Face
Thomas Haden Church: Victor Fries / Mr Freeze
Ilkka Villi: Jervis Tetch / The Mad Hatter
Manuel Garcia-Rulfo: Bane
Bill Hader: Basil Karlo / Clayface
Winston Duke: Waylon Jones / Killer Croc
Josh Segarra: Roman Sionis / Black Mask
Matt Bomer: Thomas Elliot / Hush
Manu Bennett: Slade Wilson / Deathstroke
Diego Luna: Floyd Lawyton / Deadshot
Brendan Fletcher: Lazlo Valentin / Professor Pyg
B.D Wong: Hugo Strange
Oded Fehr: Ra's al Ghul
May Calamawy: Talia Al Ghul
Robert Carlyle: Victor Zsasz
Rhys Ifans: Kirk Langstrom / Man Bat
Tom Wlaschiha: Anatoli Knyazev / KGBeast
Aaron Paul: Garfield Lynns / Firefly
Elizabeth Olsen: Andrea Beaumont / The Phantasm
Ming-Na Wen: Sandra Wu-San / Lady Shiva
John David Washington: Michael Lane / Azrael