▲ 2 r/flowcytometry
Split populations in Granulocytes
Hi
I am trying different whole blood lysis buffers to identify the most suitable one for our application. Below are a few flow plots generated using lysis buffers from different vendors (BD, BioLegend, and eBioscience; not shown in the same order). The samples were washed after lysis, and only the second sample was fixed; the others were processed using lyse/wash only.
I worked with whole blood about 10 years ago, but I do not remember seeing a split or double granulocyte population. Is it normal to observe two granulocyte populations after lysis? Is it like mature and immature granulocytes ?
u/Basic-Potential8867 — 1 day ago