IP-MS antibody failure — is phosphoproteomics a viable alternative?
Has anyone switched from IP-MS to phosphoproteomics for a low-abundance phosphoprotein after antibody capture failures? Working with PBMCs/whole blood and trying to detect a specific phosphosite via PRM after IMAC enrichment. Curious whether the switch is worth it or if sensitivity becomes the new bottleneck.
u/Constant-Rooster-372 — 12 days ago