u/Crazy-Tax-1320

Orbitrap Lumos Xcalibur. Is DIA supposed to be separate from MS1 experiment?

Hi everyone,
I’m setting up a DIA method on an Orbitrap Lumos in Xcalibur.

I want to run MS1 full scan + DIA in the same method, but I’m running into an issue:

-I can add MS1 in Experiment 1

-I can only add DIA scans in Experiment 2

-If I try to place DIA under Experiment 1, it doesn’t work or doesn’t seem supported

Is DIA actually supposed to be a separate experiment (Experiment 2) in the scan tree, or should it be possible to integrate it under Experiment 1?

Any help would be appreciated

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u/Crazy-Tax-1320 — 7 days ago