u/Dizzy-Fisherman-7858

If I'm doing label free proteomics (in any given software) for human data, what are the pros and cons of using UniprotKB reviewed proteins or unreviewed proteins as databank?

Or even for other species, it is recomendable to use a redundant (all entries) or non-redundant database for label free analysis?

As far as I understood until now, the unique peptides are important to confidentially say that a protein is present in the sample, and not it's homologous version. So in this case, would redundant entries reduce the amount of unique peptides, and thus impact the final number of identified proteins?