u/Dreaded_lobsters

I am brand new to ImageJ and using it for to measure experiments i am doing at school. I drew a rough example of what my experiment results will look like to learn imageJ. I want to measure the areas under the curved lines (area 3 in the picture). I cannot figure out why one will sometimes be picked up and the others never are recognized. Any help is appreciated.

My current method that has gotten the best results has been to make the image binary, then set thresholding to be a dark background and analysis the particles. I've been using TIFF files for the analysis.