u/Foxy_Tuba

Reviving idle Fortessa

Reviving idle Fortessa

I'm trying to help another department revive a long-idle Fortessa X-20, which sat untouched for >6 months and now doesn't work. It seems that sheath fluid makes it into the side panel, but not all the way to the flow cell. I can see that the flow cell is mostly full of air most of the time. I suspect salt crystals blocking sheath somewhere along the path, and am planning to replace all the narrow tubing and tiny T-connectors inside that I can reach. I've put some annotated pictures. Please hit me with suggestions. Eventually, I'll have to give up and tell them to call BD, but they're reluctant due to cost for an instrument that wasn't under contract and hasn't been touched in months. Also hoping someone can tell me whether sheath enters the flow cell through both the translucent teal and the dark blue tubing, and if not, what each of them delivers/removes from the flow cell.

https://preview.redd.it/h2zkm6nnxx8h1.jpg?width=1500&format=pjpg&auto=webp&s=a8fa816cfe3e7f6700150ce50cbb73af03d26b34

https://preview.redd.it/letzs7nnxx8h1.jpg?width=1500&format=pjpg&auto=webp&s=8c98cee29b3f1b39159df66c8817f39759ed8fe4

https://preview.redd.it/l9unx8nnxx8h1.jpg?width=1500&format=pjpg&auto=webp&s=30c9ce12067d7e4408ed1312373d9a04e367be5e

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u/Foxy_Tuba — 14 days ago