Hi everyone! Hope you're doing well.
Genuinely a complete beginner at Image J. So any and all insights will be very welcome. Thank you!
I've done an experiment on differentiated SH neurons, where the images I've uploaded are the control (untreated) and the other Nocodazole-treated ( among 7 other experimental conditions), these have also been stained with DAPI for nucleus, actin and tubulin. I'm hoping to measure and compare the fluorescence intensity of actin and tubulin across these conditions. I also hoping to measure the difference in morphology ( like area, neurite length, aspect ratio) with how these drug treatments affect those too.
To measure the neuron itself, I've been manually drawing around the neuron and measuring the area, and from this I have been also been able to get the aspect ratio too.
But, I've been mainly struggling to work out an accurate way to measure difference in actin and tubulin fluorescence intensity and neurite length? Any advice would be appreciated!
Thank you :]