SDS-PAGE ladder moves slower than protein samples
this is my 4th time doing sds page and my ladder is somehow always slower than my protein samples. i did some research and read that it may be due to the molecular weight or something? i would appreciate if someone could explain this to me and troubleshoot cuz im doing my undergrad and this is the first few times performing it for my FYP. if its relevant, each well contains 10microL of sample and the ladder is 5microL
edit: my protein ladder is basically moving slower than my samples to reach the dye front and i dont understand why. i know this because it was pointed out in a meeting with my supervisor that my bands for the ladder appear too close together and i was expected to experiment with the timing for that. however in my redo, i allowed the dyed sampled to diffuse into the running buffer after running for too long and my ladder also appeared “smudged” at the bottom, containing around 3 bands that were not properly separated. it might be a gel technique error but i want some advice on this thanks 🙏🙏picture of sds page for today
