u/InevitableBox0

Hi everyone.

I've downloaded and worked with multiple scRNAseq datasets without problems using prefetch, fasterq-dump, etc. But there's a dataset I'd like to work with that isn't working in my pipeline. Fasterq-dump gives an R3 file instead of R1 and R2, and I can't find barcodes in the file. It seems to be intertwined and processed with sharq.

I can't find any metadata files. However, I found bam and bai files, but when I download the bam it gives a all_contig.bam.1 file.

Is this normal? Or is it possible that the authors scrambled the data to make it unusable to others?

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u/InevitableBox0 — 25 days ago