u/Jack_O_Melli

Hi everyone!

I'm having some curious issues with the downsample plugin on FlowJo 10.10.1.

I need to downsample CD4+orCD8+ population from fcs files across 3 groups (spleen, blood and tumor). When I apply it on blood and spleen groups, it works well producing the downsampled population for each fcs file and with the correct number of events (1262 based on sample with less events).

When I run it on the tumor group it works only on some fcs files and produces downsampled population with a variable number of events different from what I select.

How to resolve it? I restart the program hundreds of times