u/Live-Hope4106

Hello!

Does anyone know the actual flow velocity for a BD FACS ARIA II system (or similar sorting system). Does anyone know what the minimum you could reasonably achieve by lowing the sheath pressure is? This is for considering actual illumination time of the cell.

I can make an estimate of ~2 m/s by using the 10 micro litres per minute quoted for flow rate 1 and and the roughly 10 micron diameter core I could see with the microscope.

Thanks very much!