u/Livid-Adeptness6021

Simple question, anyone detected this fluorescence protein before? I got a plasmid that should constitutively express mIFP, but after transfection, no fluorescence can be detected in all channels in the far red laser in bd symphony s5 ae. The same cells has been transfected with gfp and cfp with high transfection rate. Plasmid sequenced no problem…