Help a novice
Context
I’m a bioengineering student that happens to like bioinformatics and is entering this world. A professor of mine offered me to help him in a project of antibodies. The sequences of the mentioned antibodies were sequenced with Miseq Illumina from the results of a rtPCR (this was in 2010 or so). Millions of reads with only 100 bases each read. The antibodies passed panning and Elisa assays, so I have a “selection” of antibodies.
The struggle
I can’t do a de novo assembly because I have no such computing power. I know that DADA2 and QIIME2 are used for metagenomics/metabarcoding and such (remember I am very very new to this world), but I’m very interested in using ASVs to infer CDR3 regions of the antibodies and finding abundance and diversity of each one (given that that is my main goal). I know my workflow is very crooked or I may sound like I have no idea, because I don’t have any.
Any tips? I’m not looking for a complete answer but maybe for some guidance. Thank you!!