I am in Ecology/Evolution field, so if anyone has any recommendations or any journal clubs I can join online, that would be great!
It’s exactly as the title suggests. I don’t think my personality meshes well with my PI. Some labmates like them, and admire his values (that I honestly don’t see…but that’s me) but personally, I don’t see anything in them that makes me think they will be a good mentor in my gradschool life. My definition of a mentor is basically someone who will guide me to become a better scientist, a scientist who still finds research interesting after all the chaos that is gradschool. I am early into grad school, and frankly, I don’t think that my current PI will make me want to continue science. And that’s fine- I can find ways to still find science more interesting, and although rare there are people who make science still interesting so I’m holding on to that.
Anyway, to be fair, I do acknowledge my current PI’s work ethic, appreciate how they are steering my direction, and giving me enough challenges that help me improve career-wise.
But personally, I don’t like their personality. I don’t like the way they talk to me (they act annoyed when I say I don’t understand what they’re saying) so I avoid small talk and just talk about work with them. It’s fine, and it’s definitely something I can handle (for now…and later too I hope…)
I’m just wondering if you think it is a red flag? people would say to escape right away, but I also came from another lab that is the exact opposite. I had a good relationship with my previous PI, and we are actually friends, but soon enough that caused friction, because I chose another path and they took it personally.
What would be a realistic “balanced” relationship (professional but not soul sucking, friendly but with boundaries) with not only PI but other people you work with look like?
In my understanding, to make a construct for RNAi, I need to:
Am I understanding it correctly?
Also, I’m just wondering in Step 1, what are the tools I can use to do it? I saw some people use Pfam or InterProScan. Is it basically manually select regions (>300bp) that is unique to the sequence of interest, and then copy that part of the sequence to design a primer with? Also, does it need to be a continuous sequence range or is it possible to pick and choose regions that are not conserved? (Please correct me if I understood something wrong or if this is not possible)
Any suggestion or corrections will be greatly appreciated, thank you!
I’ve heard that some museums have their own PhD programs and that museum can be another place where I can do research. I’m just wondering how is the work environment/culture in museums?
In other words, what’s a day in a life of a scientist who works in a museum?
I’m thinking Natural History Museums who might have this role..?