u/Szandika11

Hi everyone! I would like to ask for your help! I need to learn how to use a flow cytometer. My only problem is, that I don't have anyone on the department that can help. And the machine that I'm using is old. Like... they don't even produce it anymore old (partec sysmex ml). And the software that I should use is the same... Flowmax 3.2. Even google corrected me that I'm not looking for this.

So I'll have to measure cells between 15-40 micrometer. And I have no clue how to set up a gain for that or a lower threshold.

If anyone can help, I would be forever grateful for that!