▲ 2 r/flowcytometry
Seeing different Forward Scatter (FSC) profiles for the exact same sample when using different stains
Hi Everyone,
II’m running into something confusing with my flow data and hoping someone here has seen this before.
I’ve been seeing that, for the same bacterial spore sample, under different staining conditions (unstained, PI only, Syto 9 only, and PI-Syto9 double stain), the FSC:SSC profile changes depending on the stain I use.
I'm thinking, would it be because the stain changed the refractive index of spores? is this something normal?
Would love to hear from people. Thanks in advance!
u/WednesdayMorning10am — 15 hours ago