u/esredditer

Hiya,

I'm really new to flow cytometry and wanted some help with normalizing and representing my cell counts. I stained my cells with PI and now my supervisor wants me to plot the cell counts of PI-positive cells to show %killing. However, I'm not sure what formula I should use to normalize these values? My supervisor said to transform counts into percentages- by dividing- Experimental cell count / control cell count *100. But in my head, this works if cells were live and the control was normalised to 100. For %killing, the control becomes 0. So, how would this work? I would have a fold change of over 200 (eg. if experimental cell count was 32,070 and control was 13,089)?

I'd appreciate any help I can get!