u/just4fun420blazeit

Hello,

We have Thermo Fisher Altis TSQ II MS. In my previous post I talked about inconsistent data post-preventative maintenance (PM). I have now realized that what I’m calling “data” post-PM are just artifacts with intensity scale 10^0 or 10^1 whereas the real analyte peaks are 10^6. I also just realized that the divert valve to my MS from LC is not connected. See the attached picture from Chromeleon showing valve “NC” which I believe means not connected. Moreover, in the command area, it also reads DivertValve A properties “DV_NOT_CONNECTED”. Subsequently, when I changed instrument method for my sequence to DivertValve A “1-2” (which is our flow to MS), it gave me an error that DivertValve is not connected.

I checked instrument controller/configuration tab but there Divert Valve is set to A so I’m not sure why Chromeleon is not picking it up.

Is this a hardware or software issue? I am already contacting Thermo about it but they’re soo slow. Any help would be appreciated!

EDIT: the field service engineer also updated our Chromeleon to the latest version during PM (our version was like 4 years old).

Hello,

In my lab we have Thermo Fisher Vanquish HPLC + TSQ Altis MS. We normally run a system suitability sample (SSS) before our real sample analysis and today, the SSS gave extremely inconsistent data even though the injections were from the same vial.

Last week, we had LCMS preventative maintenance (PM) done where the field service engineer at the end did recalibrations with the CalMix and everything looked good.

Before the PM, the data from the SSS injections was very consistent, with less than 5% coefficient of variation. Today, post-PM, everything is a mess. The peaks from the injections from same vial are eluting at different retention times, inconsistent intensity, etc.

The Tune file in Chromeleon checks everything green and the temperature, pressure, vacuum etc all look stable. There were a few leaks as well from different Viper connections but those were handled.

Any leads would be appreciated!

Thermo TSQ Altis Valve Issue

Inconsistent LCMS data post PM