u/nephhila

MALDI-TOF spectrum analysis

hey! long story short, I'm a undergrad student and I'm researching better ways to identify filamentous fungi on MALDI-TOF since they're much more difficult than bacteria or yeasts. I've run some tests and wanted the opinion of u guys how do the spectra looks, if it's good or too noisy, etc. since I don't understand what's considered to be good when you're analyzing proteome on a running metabolism instead of purified compounds.

ps.: don't mind the red identification, i already was like 99% sure the database wouldn't match since they were isolated from different environments.

https://preview.redd.it/b1cnbv3xfaah1.png?width=1920&format=png&auto=webp&s=5ac0a3ddd43459882ef36e22169e1e337377a2ab

https://preview.redd.it/vcumbv3xfaah1.png?width=1920&format=png&auto=webp&s=eefc2a67f610a575bce3787e6f1b0c2d79b303d2

https://preview.redd.it/zdxn1w3xfaah1.png?width=1920&format=png&auto=webp&s=6ddb6650e616d27a15122dee7fee0b2b02c90563

reddit.com
u/nephhila — 7 days ago