u/scienzest

▲ 24 r/labrats

Grant ended and need to cut costs. Looking to make DMEM/F12 from powder mix but can't find the formulation we use

My grant just ended and with how things are going at NIH I'm trying to extend how long I can keep my lab going until I can secure another grant. I currently use Gibco DMEM/F12 with Glutamax and phenol red, no HEPES, high glucose. Closest I've found is this product by Corning but the rep says they don't sell it in the US. I can't seem to find a mix that doesn't include either L-glutamine or HEPES and I'm trying to avoid switching to a media that doesn't use L-alanyl-L-glutamine. Hoping someone here may have suggestions on where to find this or something close that we can supplement.

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u/scienzest — 1 day ago