Here is a one-person Journal club covering a really great paper that showcases both CRISPR/Cas9 but also P2A sequences to allow tracking of expression in transient transfections.
See also: The publication in Nature Communications
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After diagnosing many CRISPR/Cas9 candidates over the last 10 years I have encountered some phenomena that people don't seem to talk about. This video is me giving a name to these and indicating why they are not only worth considering but celebrating too.
The cover image was generated by AI. The other images are from published papers on which I am a middle author.
If you take the time to watch the video then listen to my soft Scottish accent and unconventional manner of speech and ask yourself if there is an AI on earth that could generate that.