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▲ 36 r/DIYbio+1 crossposts

We developed a 3D B-cell spheroid model to mimic aspects of secondary lymphoid organs

We recently published an open-access study describing a 3D co-culture model of human B cells and CD40L-expressing stromal cells using plates coated with N-hexanoyl glycol chitosan. The system supports spheroid formation, B-cell differentiation, and class-switch recombination, providing an in vitro approach to model aspects of secondary lymphoid organs.
I thought this might be interesting to researchers working on B-cell biology, immunology, biomaterials, spheroids, or 3D culture.
Happy to share and discuss the work!

https://doi.org/10.1039/d5bm01039j

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u/Sea-Friend-1056 — 12 days ago
▲ 2 r/DIYbio

Selling Blood Coagulation Analyzer for use or for dismantling an recycle in bio projects

  • The Dade Behring / Siemens BFT II (Behring Fibrintimer II) is a semi-automated, dual-channel coagulometer designed for routine and specialty blood coagulation testing in low-volume laboratories. The detection principle combines mechanical and photo-optical methods using a turbodensitometric detection method, which allows for accurate measurement of lipemic (milky) and icteric (jaundiced) samples.
  • The unit was removed from a laboratory in working condition. I am unable to perform a complete analytical test because I do not have the required reagents or compatible printer paper. 
  • The device powers on normally, with no abnormal fan or internal noise. The RAM and ROM self-tests are completed successfully, and the unit heats to a stable operating temperature. All functions appear correctly on the display. Once fully heated, pressing the selection key allows the unit to enter the measurement mode without errors. It auto blanks and incubates cuvettes successfully once a measurement mode is selected.
  • A free 3D-printed solution for the printer paper spool holder can be provided, together with the available user manual and technical documentation.
  • Open to negociations/offers
u/Legal_Life3468 — 10 days ago
▲ 0 r/DIYbio+2 crossposts

Five great movies about cloning

Here are my five favourite movies featuring cloning

This is not a definitive list but all five are worthy of note.

youtu.be
u/Novel-Structure-2359 — 11 days ago