r/MedTechStudentsPH

random story time, pero ano gagawin niyo kapag may naobserve kayo sa mga staff niyo sa lab na may kakaibang nangyayari? merong 2 na alam naming may karelationship na and public sa soc med pero palaging inaasar kahit marami kami sa paligid. May mga sinabi pa ata si staff A (Male) regarding kay staff A (female) na bet niya daw si Ma'am at "cute" daw lol satrue lang sobrang cringe na may ganun pala talagang tinotolerate yung cheating sa workplace? si Ma'am A mukang okay lang din sakanya kahit may bf siya. may mga recent pics pa silang dalawang shiniship na magkatabi. nakikita din namin usually yung gf ni Sir A pumupunta sa hospital nagaantay sa lobby and nakakaawa na hindi mo maintindihan kasi posibleng di niya alam or nagpapakatanga siya? may ganun pala talaga. hindi ba nakakaconcern sa management yung ganung scenarios? okay namang magmind your own business pero ano ba thoughts niyo sa mga ganyang kaganapan sa lab? as a soon to be RMT parang nakakadiscourage lalo na ambaba na nga ng magiging sahod natin may kabitan pa parang nakakababa ng class ng lisensya natin yung ganun 😖 sa totoo lang balak na namin imessage yung gf ni Sir pero baka magcause ng gulo. ano advice niyo?

Hi! My groupmates and I are currently conducting our undergraduate research, and we're having trouble with our antimicrobial susceptibility test (AST) using a plant extract.
We're following the methodology in our research paper, but we're consistently getting no zone of inhibition (ZOI) from our plant extract, while our positive control works as expected.
This is the procedure we're following:
Colonies of Pseudomonas aeruginosa grown for 16–18 hours on Brain Heart Infusion Agar (BHIA) are suspended in sterile distilled water and adjusted to a 0.5 McFarland standard (~1.5 × 10⁸ CFU/mL).

Mueller-Hinton agar (MHA) plates are inoculated by streaking the standardized suspension in three different directions to ensure even distribution.

Sterile 6-mm paper discs are impregnated with 20 µL of the plant extract, allowed to stand briefly, and then placed on the inoculated MHA plates using sterile forceps.

Ciprofloxacin is used as the positive control.

All treatments are performed in triplicate and incubated at 37°C for 24–48 hours.

After incubation, the plates are examined for zones of inhibition.

Despite following this procedure, our plant extract consistently produces no inhibition zone at any concentration.
Before we change our methodology, we'd like to ask:
Is there anything in this procedure that should be modified?

Could the issue be related to the extract concentration, solvent, disc loading volume, agar diffusion, or another factor?

Would you recommend a different method for testing crude plant extracts?

Any suggestions or insights would be greatly appreciated. Thank you!

Hello all,

I will be departing with my senior co-workers next month and would like to compile best gifts(mini) for medtechs. Preferably things they could use often and will not accumulate dust. Size of the bag is palm sized mesh bag , so any ideas are appreciated. I only have a personalized keychain with their names atm.

Thank you.