r/tissueculture

Hello Everyone! I'm new here and would appreciate it if someone could give me any advice on rooting hormone.

This is my Datura metel culture~

Datura metel from leaf explant (media comp: MS + ABA 1 PPM)

I initiated the callus from leaf explant using BAP and NAA then it stucked on callus phase. Someone suggested that I add ABA, and yay! The leaves formed nicely!

Now I need to induce some roots, so does anyone have any recommendations on which hormones I should add? Or should I keep it in ABA to induce more shoots first?

Any help would be greatly appreciated!