Just wondering if anyone has experience with this? I've heard some food coloring will degrade from light which would suck. These colors were all a bit more vibrant before autoclaving. I'm just trying to get away from endless labels for different media mixes.
Something less divisive to share 😁.
Some Pereskiopsis a few weeks into culture first generation explants.
So far we found they grow short dark green on full MS with .5 mg BAP.
Well on 1/2 MS cactus media they shoot much better with almost twice the size in the same time.
Some extra cactus pictures on 1/2MS with 2% sugar.
The first three pics are different angles of what im assuming to be a jade leaf callus? I just cut it up today to propagate onto new media and upon cutting it reminded me of broccoli.
Last two pics are of a Lophophora species, a type of globular cactus, and it grew some roots and an off shoot of some kind along with some weird underdeveloped stuff near the center.
These were put on the same media using a 1:1 ratio of NAA and BAP. Just curious as to what kind of growth this hormone ratio produces, I know it varies among species but is there any general trend?
Any input/advice is appreciated!
Washed and placed on
MS media
Half MD media
DKW
Andersons Rhododendron media
Growth on most of our explants, but Andersons Rhododendron mix and Half MS seemed to grow the fastest. Next week we will sub culture and test different hormones for rooting.
NAA
IBA
IAA
I couldn’t find anything in the group rules about sales. I have a business selling tissue cultures and also have my own in house lab where I propagate plants too. I primarily sell carnivorous plants and begonias.
I have two tissue cultures currently in the container. They’ve been in there for about three days. Today I noticed they weren’t looking so good and realized the holes at the top were open all the way. I think it’s been like this for less than 24 hours, but it’s still done some damage. I’ve closed the top, but wondering if anyone has any tips to making sure they don’t fully die? How long should I wait to open it a little bit? Should I spray some water inside to create humidity again? Any help would be appreciated! Thank you
Hey guys I haven’t gotten my TC yet and I’ve never done it before but I ordered 20 😭
Does anyone use rooting hormone when acclimating TC? This is the one I have
The exponential growth will always amaze me. From 20 or so sprouted seeds we are now in triple digits. Moving them to multiplication tubs. Should be ready for production before the end of the year.
It's very small, but it has everything I need
I have some rare carnivores I wanna get into TC to preserve genetics … get at me! Thanks!
Both my tissue culture plants and slumped over and one has brown leaf, are they both cooked?
What do I do?
Do I cut the leaf?
Can I save them?
Hi all, just wanted to see if anyone has ever TC a chiloschista or any other leafless/ghost orchid? I can’t seem to find a protocol for it anywhere