r/tissueculture

Image 1 — Been a little slow!
Image 2 — Been a little slow!
Image 3 — Been a little slow!
Image 4 — Been a little slow!
Image 5 — Been a little slow!

Been a little slow!

Life got busy & some of the projects have been on idle.

Getting a much needed vacation next week and I'm finally at a point where I should have more time when we get home!

Excited to dive back into researching and hands on learning again. Will finally have a chance to apply a lot of the new knowledge and ideas that I've been rolling around.

Do have some cool stuff still cooking though! A few Lophophora & copiapoa humilis seedlings on media in 50ml centrifuge tubes. Going to transfer these to 16oz deep media vessels in a couple weeks.

u/GreenhouseGasPlants — 1 day ago

Kratom update

Learning valuable lessons, just because your plants are alive and growing doesn’t mean they are thriving.

Changed up are nutrient media and had healthy roots in 5 days with no PGR’s.

First picture is MS with hormones after a month. Second picture is McCown woody media with no PGR’s after 6 days.

u/EthnoBotanicalsTC — 3 days ago
▲ 105 r/tissueculture+2 crossposts

Pineapple tissue culture – Multiplication stage

Hi everyone!

I'm an Agronomy student from Brazil, and I've recently started documenting the plant tissue culture work we do in our university laboratory.

This video shows the multiplication stage of pineapple (Ananas comosus). The plantlet is transferred from the establishment medium to a multiplication medium, where it will continue to grow and produce new shoots over the next few weeks.

I'm documenting my learning journey in plant tissue culture, and I'd love to learn from the community. Any suggestions, tips, or feedback are greatly appreciated!

I also plan to share future work involving banana, bromeliads, orchids, and grapevine tissue culture.

u/Life-Perception-4968 — 8 days ago

Does this TC look healthy?

Okay so this is my first tissue culture it’s a Monstera white monster , I was gifted it at a plant expo exactly a month ago - had no clue at all what I had gotten into. I’ve struggled with keeping it humid enough so recently moved it to the ziplock bag it also it was not getting proper lighting before putting it under its own grow light. Stupidly enough I also didn’t put it in a clear pot… but I am worried about my plants health as I have seen zero progress, there’s is no browning on any of the leaves so it’s still alive lol? Not sure what I could do to help growth.

Any advice is welcome :)

u/Double-Breath-8134 — 5 days ago

Day 1 vs Day 21 🌱

Didn't expect to see this much progress in just three weeks. Taking weekly photos has made it easier to notice the small changes that I'd probably miss otherwise.

Still learning as I go, but I'm excited to see how it looks in another few weeks.

u/GreenLeafHelper — 7 days ago

Max number of hours under grow light?

I'd like to size up some allocasia, anthurium and monstera tissue cultures as fast as possible. I'd like to get them up to 14 hours. How old should they be before I get to 14 hours? Anyone gone through something similar what was your strategy?

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u/Excellent-Phone8326 — 9 days ago

Reliable tissue culture labs in China – happy to help source rare plant tissue cultures

Hi everyone!

I’m based in China and have been working with rare plants, especially orchids, for many years. Recently I’ve started working with tissue culture laboratories in China as well.

I can help source tissue culture plantlets and connect buyers with laboratories that offer:

* Custom tissue culture propagation
* Contract production (OEM)
* Mass propagation
* Virus-free plant production

Currently available genera include many popular plants such as:

* Alocasia
* Monstera
* Philodendron (depending on availability)

Examples include:

* Alocasia watsoniana
* Alocasia Frydek Variegata
* Alocasia Dragon Scale
* Alocasia Silver Dragon
* Monstera Aurea
* Monstera Mint
* Monstera Thai Constellation

If you’re looking for specific cultivars, feel free to send me a message. I’ll check availability with the laboratory.

I also have an established eBay store, so if you’d like to verify my seller history before doing business, I’m happy to share it privately.

Thanks!^_^

u/Jacquie-Sss — 10 days ago
▲ 12 r/tissueculture+1 crossposts

Tissue culture in perlite only ?

Hi ! I wanted to try tissue culture plants so I pre-ordered one last month and then completely forgot about it, it should be delivered tomorrow but I don't have any stratum, can it work with perlite only ? Once acclimated I plan to put it in pon.

u/Old_Most_6917 — 13 days ago

After a week, shoots are starting on the Pereskiopsis

Just cleaned this cactus last week, we will be grafting onto it in-vitro very curious to see what it can do

u/EthnoBotanicalsTC — 14 days ago

Looking for reliable tissue culture company

Hello! I am looking for tissue culture companies overseas (Asia) that carry rare tissue cultures and are reputable. I am looking for things like pink venom, sedenii pink, Polly candy, etc. I’d love some recommendations so I can start importing more of the rare tissue cultures. Thanks!

reddit.com
u/Bubbly_Low9491 — 13 days ago

Woody plants

Was asked on here recently about woody plants, started our first experiments today with different nutrient media. Does anybody have insights or advice? Has anybody used any of these mixes? Also very curious to see how they affect the PGR’s in future experiments

u/EthnoBotanicalsTC — 13 days ago

Ordering Tissue Culture Supplies to Ontario Canada

Hi all

Does anyone have recommendations on ordering tissue culture media to Ontario (GTA)? I'm looking to try tissue culture and I've noticed Phytotech labs seems to be a popular choice but I'm worried about duties and shipping fees, coming from the US. I've searched online through multiple Canadian stores though they all seem to be either reserved for corporations or selling overly generalized pre-mixed tissue culture media.

Thanks! SFD

reddit.com
u/SomebodyFixDave — 13 days ago