Overcooked tissue?
Hello all!
Been trouble shooting some difficult tissue for a while now. Testing cardiac muscle and vessel FFPE that is incubated in HIER for 30 seconds at 125c. The issue I am running into is our extremely old pressure cooker can take 20 minutes or 2 hours and usually that 1-2 hour is when we see tissue damage and no antibody binding. I have attached an image of some of the damage. Additionally the muscle fibers seem shrunken compared to H&E. We allow it no time to dry out as well so likely not that. Any thoughts?
u/1s22s22p1 — 7 days ago