r/Histology

Bonjour à tous,

Je me permets de vous solliciter car je suis actuellement en train de lire un texte historique et académique sur l'affaire Dreyfus et le célèbre « J'accuse » d'Émile Zola, et une subtilité contextuelle me pose question.

Voici le passage concerné :

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Concernant l'expression « contre lui », j'hésite entre deux interprétations pour l'antécédent du pronom « lui » :

1. Émile Zola lui-même : Zola aurait délibérément provoqué l'armée et le gouvernement pour que ces derniers intentent un procès pour diffamation contre lui-même (perspective de stratégie judiciaire). Sur le plan grammatical, le sujet de la proposition étant « un article », il serait tout à fait correct de désigner Zola par « lui ».
2. Ferdinand Walsin Esterhazy : Le pronom renverrait à Esterhazy, qui était le sujet de la phrase précédente. L'objectif ultime de l'article aurait donc été de traduire à nouveau le véritable coupable devant la justice (perspective de révision du procès).

En pesant à la fois la structure grammaticale de la phrase et la réalité historique de l'époque (notamment le fait qu'Esterhazy venait d'être acquitté et que l'autorité de la chose jugée empêchait de le rejuger directement), selon vous, quelle interprétation est la plus juste, la plus naturelle ou la plus couramment admise par les locuteurs natifs et les historiens ?

Je vous remercie d'avance pour le partage de vos précieuses lumières !

I’m over trying to get into histology. Originally,I wanted to be a pathologist assistant but I wasn’t so good with the sciences in college and didn’t want to do the extra steps to get into pa school. So I came across histology which I was interested in because it’s in the same realm and also less schooling. So I received a records management job at a bio pharmaceutical company that has a histology department. I express my interest to many including my senior manager and regular manager. They spoke to the manager of the histology department and gave me permission to shadow for a month. I loved watching the process for cryosectioning and paraffin sectioning. After the few weeks were done I asked on the last day if the department is conducting training on the job again like they did previously. They said no due to the heavy load of work. They were hiring I asked if I could apply, no I needed certification. So I looked in the histology programs online because there are none here in Jersey. I completed biology 1 but still needed chemistry. So I finally took and passed chemistry through Hartford community college. I was prepared to submit my application but of course the program costs a little over $1000 monthly. I’m still a contractor at the pharmaceutical company I don’t have it that right now. So I tried to apply to UND for this summer program but the deadline passed. The admission person said I still needed chemistry and algebra for the program.
I refuse to retake chemistry again and I just want to go into the program. I’ve been looking for grossing jobs, applying to histology jobs, and looking for specimen jobs. But they’re either 2 hours from me or require more experience. I’m clueless now. Any advice? Anyone knows if Hartford takes student aid?

Does anyone know the best temperature for cutting mice tongue tissue on the cryostat? I’ve adjusted from -22 to -11 and the OCT is constantly crumbling into pieces once it hits the blade and I’ve never had this problem before.

Do you guys consider yourselves scientists? I’ve always reserved that title for more research positions but I wanted other peoples opinions on it. My coworker consider himself a scientist but my other one says he’s not.

Hello,

I’m just making a post to see if anyone has experienced something similar. My goal is to do the Histotech program AA at Mt Sac. My registration date was May 13th but before I even got a chance to register all the seats + waitlist was full. My question is do you think I have a chance of getting into the class? I’m waiting until jun 12 to see if maybe someone doesn’t pay their fees on time & gets dropped & maybe I’m able to secure a seat this way? I also got all my general Ed done at a different community college so there are no extra classes I “need”. Thanks for the input

What is everyone’s favorite equipment in the lab? Do you have a favorite microtome,tissue processor, or cryostat?

So I originally thought I wanted to go into research (PhD track), however my current job has made me not want to pursue academia in that way anymore. Histology has always been extremely interesting to me and has the parts of science that drew me to research. My main issue is finding the most cost effective way to sit for the exam. I have a bachelors degree and have been working in a research lab for the past year, however it isn’t a “histology lab”. It covers a lot of the techniques that are required, but certain techniques seem to be done different in this lab vs what would be done in a histology lab. Has anyone else gone down “route 2” coming from a lab that isn’t considered a histology lab? I am hoping to avoid getting a masters, as I cannot afford to do so.

Hey everyone,

So, I applied for a per diem histotech position at my local hospital. I was feeling alright about this until I scheduled the interview over the phone, because they said to expect it to be 1 hour to 1 hour and a half long, and that there would be a skills demonstration.

I have no experience with a "skills demonstration" during the interview process. What should I expect? Any advice or anything I should look out for? Does someone actually sit there staring you down the entire time? 😭

When I first heard them say it, I started freaking out and almost told them never mind.

I've been working in a histology lab since 2022. For the first year, I was more of a lab assistant, accessioning and cutting frozen. After that, I really started getting into the histotech work, like using the microtome. The only issue was that I was one of the later-morning techs, and our volume was low, so most of the embedding was already done. I embedded only a handful of times at this first job.

I started a 2nd histo job about a year ago now, and have embedded more than I did at my previous job within the first couple months, and our volume is maybe 4x more than that of my previous job. I feel waaaay more confident embedding now, but I can definitely work on my speed, and my manager agrees 😅. My supervisor said I should be able to embed a whole basket (about 72 cassettes) in 30 minutes. Depending on whether the tissue is wrapped in those annoying mesh bags and papers, I can get the whole basket done within 30-40 minutes, but more towards 40 minutes.

My supervisor says my microtomy is good, but sometimes I feel like I have good and bad days cutting. Bad days, meaning I can't get my biospies to ribbon, or I have an ice full of crunchy hard tissue that feels like it takes me a day and a half to get through

moral of the story, I'm terrified of this testing part and feel inadequate

Hello everyone! I’m wondering how your lab would deal with bringing up someone’s speed during embedding? We have a (supposedly) seasoned histotech who is newish to our lab, that regularly takes an extremely long time to embed. Just the other day for example they took about 2.5 hours to embed roughly 50 blocks. We’ve brought it up to our supervisor before and all they do is complain about the person behind their back and never actually address the issue with them.

any tips to minimize tissue loss when removing coverslips? I'm staining for miRNA, and the kit I'm using suggests a coverslip during the antigen retrieval step (20 min in chamber placed in 98C waterbath)