u/kiebie69

Hi everyone of this lovely sub, thank you so much for all your advice for my previous posts. I'm now trying PAS stain on my mice livers to see if the droplets I'm seeing are lipid or glycogen deposits. Firstly in my stain i see positive staining favours one side of the cell- is that normal or is it a problem with the staining? I'm guessing the areas positive for glycogen be the more magenta areas vs the unsaturated purple? Any advice or comments on my stains are much appreciated!

Here's my protocol briefly: deparaffinize & hydrate, PAS for 8 min, rinse in tap and distilled water, Schiff reagent 20 min, 10 min tap water, haematoxylin for 2 min, acid ethanol for 5 seconds, tap water 15 min, distilled water, dehydrate & clearing