u/AlaaKD

Pan Cyan agar (weza), transparent halo around mycelium — bacteria or normal?

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The mycelium grows white with no obvious mold contamination, but growth is slow and there’s always a transparent/wet-looking halo around the mycelium. The strange thing is the halo only appears around the growing mycelium and doesn’t spread across the plate by itself.

I took transfers from the clean edge, but the same thing appeared again on the new plates (second pic, day 3).

So now I’m confused:

is this bacterial contamination hitchhiking with the mycelium?

should I keep transferring on agar or move to grain?

can cultures like this still fruit normally?

First pic = original spore plate

Second pic = transfer plate

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u/AlaaKD — 6 days ago

Pan Cyan agar (weza), transparent halo around mycelium — bacteria or normal?

The mycelium grows white with no obvious mold contamination, but growth is slow and there’s always a transparent/wet-looking halo around the mycelium. The strange thing is the halo only appears around the growing mycelium and doesn’t spread across the plate by itself.

I took transfers from the clean edge, but the same thing appeared again on the new plates (second pic, day 3).

So now I’m confused

is this bacterial contamination hitchhiking with the mycelium?

should I keep transferring on agar or move to grain?

can cultures like this still fruit normally?

First pic = original spore plate

Second pic = transfer plate

u/AlaaKD — 6 days ago

Any successful attempts to grow MIB in a shoeboxe without Martha tent?

I'm about to start my first pan grow. I don't have a Martha tent but i could put a humidifier in the room if necessary. I was searching for the most forgiving strains to start with and i heard that MIB and Weza are the ones. If you have any successful attempts to grow these without Martha tent, please share with me.

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u/AlaaKD — 16 days ago

Hey everyone,

Put Weza spores to agar 7 days ago. A clear transparent bacterial film formed around the spores almost after 3 days, stopping any horizontal spread.

3 days ago I started noticing this pale, wispy, growth shooting straight UP from the bacterial spots (pics attached). Zero horizontal growth.

Is this Cobweb mold, or could it be aerial Weza mycelium desperately trying to escape the bacterial surface?

Should I attempt a T1 transfer from the very top tips of these hairs, or toss it? Appreciate any advice! 🍄

u/AlaaKD — 21 days ago

I've only tried pans once and it was a wild one from Indonesia. The trip was amazing, euphoric, clean, crisp, light and no body load at all.

I'm starting to grow weza now and i heard it's not a pan cyan strain but is similar.

I want to hear your trip reports on it and how you compare it with other strains. Is it the same clean head space, no body load experience as the other pans?

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u/AlaaKD — 26 days ago