u/Dense-Flight9663

LTQ XL CALIBRATION KEEP FAILING

Hello everyone,

I've been having a lot of trouble with the calibration of my LTQ XL. Below are the results I've been getting. Could someone please help me understand what might be going on and what these results indicate?

I'd really appreciate any insights or suggestions. Thank you!

CALIBRATION RESULTS:

Checking Multiplier # 1 Calibration for Positive Ion Mode

19:49:06: Finding gain at multiplier voltage = -1247 v

19:49:40: Multiplier Gain OK: gain = 423622

19:49:40: Finding gain at multiplier voltage = -1125 v

19:50:24: Multiplier Gain OK: gain = 99323

19:50:28:

19:50:28: Checking Gain Ratio for Multiplier 1

19:50:41: Results of Multiplier Check:

19:50:41: High Gain Signal = 474442059

19:50:41: Normal Gain Signal = 17998900

19:50:41: High Gain Signal/Low Gain Signal = 26.360 with RSD 38.248%

19:50:41:

19:50:41: Ratio result is not within expected range

19:50:41: Retry measurement

19:50:44:

19:50:44: Checking Gain Ratio for Multiplier 1

19:50:58: Results of Multiplier Check:

19:50:58: High Gain Signal = 512991299

19:50:58: Normal Gain Signal = 24901413

19:50:58: High Gain Signal/Low Gain Signal = 20.601 with RSD 27.303%

19:50:58:

19:50:58: Ratio result is not within expected range

19:50:58: Retry measurement

19:51:02:

19:51:02: Checking Gain Ratio for Multiplier 1

19:51:15: Results of Multiplier Check:

19:51:15: High Gain Signal = 485089028

19:51:15: Normal Gain Signal = 26911565

19:51:15: High Gain Signal/Low Gain Signal = 18.025 with RSD 30.176%

19:51:15:

19:51:15: Ratio result is not within expected range

19:51:15: Positive Ion Multiplier Gain Calibration FAILED

19:51:16:

19:51:16: SUMMARY of CALIBRATION:

19:51:16: Positive Ion Multiplier Gain Calibration FAILED

reddit.com
u/Dense-Flight9663 — 27 days ago

Mass spectrum messy after CID

Hi everyone
I have been experiencing a messy mass spectrum after CID especially MS2. Does anyone know how to resolve this?

u/Dense-Flight9663 — 2 months ago