▲ 2 r/NMN_NR_NAD+1 crossposts

Running NAD+

Hey everyone,

I’m looking for some feedback on my current NAD+ protocol and whether my planned schedule is sustainable long-term.

For the past 10 days, I’ve been doing 60mg NAD+ injections daily (subcutaneous/IM). So far, I've handled the acute flush/side effects completely fine, but I know running a consecutive-day schedule this long is pushing past a standard loading phase.
My original plan was to keep running this either 5 days a week (Monday–Friday) or even 7 days a week continuously.

A few questions for those who have experience with long-term, high-frequency NAD+ cycles:
1 Is 5–7 days a week indefinitely too much? Am I going to hit a wall with receptor desensitization or diminishing returns? Or should I pivot to a standard 3x a week (M/W/F) maintenance routine now that my first 10 days are done?
2 Methylation Support: I know running this much NAD+ heavily drains the methyl pool. I haven't been supplementing with a methyl donor yet, but I'm planning to pick up some oral TMG (Trimethylglycine / Betaine Anhydrous) to take on injection days. What daily oral dose of TMG are you guys pairing with a \~60mg injection protocol?
3 Any specific brands of TMG you recommend, or things to look out for regarding mood/sleep changes when pushing a high-frequency
schedule like this?

Appreciate any insights or anecdotes from anyone who has run similar daily protocols!

reddit.com
u/Environmental_Oil144 — 2 months ago
▲ 4 r/NMN_NR_NAD+1 crossposts

NAD+ injections

Hey guys just started NAD+ today 50mg injection to back of arm. I’m also on 2mg weekly Reta.

My research suggested 50mg 3x a week. Does this sound right or should I go based on my reaction to it?

Don’t feel any side effects as of yet but I just pinned it 20 min ago

Thanks

reddit.com
u/Environmental_Oil144 — 3 months ago
▲ 1 r/NMN_NR_NAD+1 crossposts

NAD starting dose

Hello. I started taking NAD+ today with 50units / 0.5ML

Is it too high? If so, should I take the next interval by 25units instead?

Take note that I will inject 3x weekly.
I had NAD+ 500mg with 5ML BAC
Please let me know your thoughts.c

reddit.com
u/Environmental_Oil144 — 3 months ago
▲ 20 r/NMN_NR_NAD+2 crossposts

Very fast delivery, Fifty410/Quaker- NAD+

I placed an order for NAD+ on 6/2 around 11:00 AM using the Offerings tab in the Fifty410 portal. I paid within about an hour and shortly after received confirmation that the prescription had been sent to Quaker Community Pharmacy.
FedEx picked up the package from Quaker about an hour later, and it arrived at my doorstep in California less than 24 hours after I placed the order.

Packaging was solid: Nordic Ice packs inside a foil/foam insulated mailer, everything was still cool on arrival, and the package included individually wrapped syringes and alcohol prep pads.

The NAD+ is compounded by Annovex (a 503B facility). I wasn’t familiar with them before this order, but the product details are:
6 mL vial
200 mg/mL concentration
1,200 mg total NAD+
BUD of 10/4/26

Overall, I’m extremely impressed with the turnaround time and service. This order was actually a last-minute backup because my usual NAD+ and glutathione bundle refill from Gimme has been delayed for over a week at Greenwich Pharmacy. Fortunately, this worked out perfectly and got to me incredibly fast.

u/Environmental_Oil144 — 3 months ago
▲ 4 r/NMN_NR_NAD+1 crossposts

NAD+ Injection Sites

What is the most effective injection site you’ve all seen for NAD+?

FYI: I’m new here so please don’t crucify me. I’m also aware of all of the basic injection sites and such. I’m asking for personal experiences and preferences. Thanks for letting me be a part of this!

reddit.com
u/Environmental_Oil144 — 3 months ago
▲ 20 r/Renue+1 crossposts

STUDY: New Human Data Challenges the “Universal NAD+ Decline With Age” Model

A new review of recent human NAD+ research argues that the classic narrative, “NAD+ declines with age”, is probably too simplistic.

Key Points

• Whole-blood NAD+ does not consistently decline with age in newer studies
• Tissue NAD+ (muscle, skin, brain) often does decline with aging
• Physically active older adults can maintain youthful muscle NAD+ levels
• NAD+ response to supplementation varies substantially between individuals
• Blood NAD+ may reflect intervention response better than biological aging itself

This is an important shift in the field. The newer data doesn’t invalidate NAD+ biology but gives it new nuance. The emerging picture is that NAD+ appears to be:

• Tissue-specific
• Highly dynamic
• Influenced by health status, activity level, and disease burden
• More individualized than previously thought

This is a reminder that biomarkers measured in blood don’t always represent what’s happening inside active tissues.

Full Article: https://renuebyscience.com/blogs/scientific-evidence-library/nad-aging-blood-biomarker-human-study

u/Environmental_Oil144 — 3 months ago