u/Hot-Consequence-2286

Hi r/bioinformatics, I've been frustrated with the complexity of qPCR analysis tools, so I built a browser-based alternative that runs as a single HTML file. What it does: - Standard curve validation (R², efficiency, slope) - ΔΔCt expression analysis - Housekeeping gene stability (geNorm M-value) - Welch t-test + ANOVA + Benjamini-Hochberg FDR correction - Automatic outlier detection - QC report with pipetting precision heatmap - Publication-ready PNG export - Works offline — no install, no account, no subscription Try the demo here: https://KmBioChemo.github.io/qPCR-Analyst/ Full version is 9€ one-time purchase.

https://preview.redd.it/z930xm9n8w2h1.jpg?width=1267&format=pjpg&auto=webp&s=a00dddf4da5a8fe1824f59a68d9f31ab4aaeec13

https://preview.redd.it/518joxln8w2h1.jpg?width=1134&format=pjpg&auto=webp&s=3e548e60ef255e0e0f3d65a728f85df9fb833dc5

https://preview.redd.it/64iq2sgo8w2h1.jpg?width=1121&format=pjpg&auto=webp&s=22ce41ab5c99954428065ea9f95f8ceb72e2936d

Hi r/bioinformatics, I've been frustrated with the complexity of qPCR analysis tools, so I built a browser-based alternative that runs as a single HTML file. What it does: - Standard curve validation (R², efficiency, slope) - ΔΔCt expression analysis - Housekeeping gene stability (geNorm M-value) - Welch t-test + ANOVA + Benjamini-Hochberg FDR correction - Automatic outlier detection - QC report with pipetting precision heatmap - Publication-ready PNG export - Works offline — no install, no account, no subscription Try the demo here: https://KmBioChemo.github.io/qPCR-Analyst/ Full version is 9€ one-time purchase. Feedback welcome!

https://preview.redd.it/yjylksqz8w2h1.jpg?width=1267&format=pjpg&auto=webp&s=de9d693b419f25cd628f58f19adc11caea89df11

https://preview.redd.it/3nascg409w2h1.jpg?width=1134&format=pjpg&auto=webp&s=0488baae0b7f31365cb966f0ca926d3a0b7b8a11

https://preview.redd.it/nzr7pdk09w2h1.jpg?width=1121&format=pjpg&auto=webp&s=a7ce76db7204fa2e069131ca3d9df6efbb7edf19