r/molecularbiology

Hey guys, I have 12 plates to run over the next couple days and was wondering if it's possible to store my master mix (SYBR, nuc free water, primers) + template overnight ? If not, is it possible to store just the master mix overnight ?

PS: my cDNA is extracted from urinary RNA, so I expect yield to be quite low

I am looking for podcasts about genetics and molecular biology or neurology and I would love some recommendations.

Hi everyone,

I’m currently doing my Master’s in Biotechnology, with a particular interest in molecular biology and bioinformatics. I also have a few short-term work research experiences.

For those working in the biotech/pharma industry:

1. What roles are you currently doing, and what does your day-to-day work look like?

2. Given a background in biotechnology + molecular biology/bioinformatics, what roles would you recommend I target?

3. Which entry-level roles are relatively easier to get into after an MSc?

4. How difficult was it for you to get your first proper industry job?

5. What skills or experience helped you get hired?

I’d especially appreciate experiences from people in the UK/Europe. Also, if you could mention your degree, current role, years of experience, and how you got into the industry, that would be really helpful.

Thanks!

Specific application I have in mind: Plant and mushroom identification for foraging, and propagation.

Hi everyone,

I'm in the research discipline of molecular microbiology and infectious diseases, and I've recently been given the opportunity to visit the National Institute of Infectious Diseases, Tokyo, Japan, to receive hands-on training in Sanger sequencing and other molecular laboratory techniques.

Since the training period will be one month, I'd really appreciate advice from people working in molecular biology, microbiology, sequencing or related fields:

If you had access to a well-equipped research institute for a limited period, which laboratory techniques would you prioritise learning?

I'm particularly interested in techniques that would make me more independent and versatile as a researcher, rather than simply learning techniques for the sake of having them on my CV.

Also, if there are any techniques that you learned early in your career that ended up being unexpectedly valuable, I'd love to hear about those too.

I thank everyone in advance

Hi, I'm designing a website where biologists can delineate the borders of habitats that determine where residents get to vote on issues relevant to their home habitat. I am at the stage where I need to test out the verification process for biologists empowered to vote on where borders of habitats should be. Can an accredited biologist please sign up to the website and go through the verification process for me? It requires that you link two images of your certificates. Thank you!

This is related to Cascadia because this website will help make Cascadia, a habitat region, a reality.

https://primaryhabitat.pythonanywhere.com/

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I am loosing too much DNA amount after gel extraction. Can anyone suggest how can I increase the yield of DNA after gel extraction? I am using Qiagen kit

Hi everyone,

I’m expressing human βA-, βS-, and γ-globin chains individually in S. cerevisiae and detecting them by SDS-PAGE/Western blot.

I came across a paper on the thermal behavior of hemoglobin that found irreversible aggregation above 55°C. Since standard SDS-PAGE/WB procedure is heating the sample to 95°C, would heating globin samples at that temperature cause aggregation and maybe affect the Western blot signal?

Would it make more sense to use a lower temperature, such as ~72°C, for sample preparation? Has anyone worked with globins or similar proteins and compared different heating conditions before SDS-PAGE?

I am an international student that hit a financial roadblock. I’m not able to afford the last semester of my undergraduate biology degree so I have decided to go all in and graduate a semester early. My schedule is absolutely packed with classes, work, and a thesis.

My research was initially very important to me because I had plans to do research work/go to grad school. But with 24 hours on my class schedule, I’m not sure it is worth it.

I have research experience on my resume with a literature review in progress, a 2 month long plant transformation as well as a summer yeast transformation independent study. I’ve heard how important research experience is in finding a job in biology. My question is whether or not this alone is sufficient to finding a job, any job, in biology or getting accepted into grad school.

Any advice would be so appreciated.

Hi! I work as a PCR test developer and have run into a problem.
My experience isn't sufficient to resolve it, so I’m posting here. Background signals have started appearing in my system. When using a primer-to-probe ratio of 3:2.5:3, I get weak curves (up to 50 RFU), yet the test system fails to detect samples with concentrations below 1,000 copies/mL.

We order our primers and probes from GenScript. Can anyone help?

Here are my five favourite movies featuring cloning

This is not a definitive list but all five are worthy of note.

I've always been fascinated by how proteins actually work and how their biomechanics enable them to do their tasks. So, I've made this interactive article that builds up hemoglobin part by part, using tailor-made 3D models and animations to show how its structure and mechanics allow it to grab oxygen at the lungs and let it go near tissues. I also cover the Bohr effect, cooperativity, 2-3 BPG, and a brief history to highlight the work of scientists that contributed to the discovery. Feedback and corrections welcome!