u/Hot_Love8969

Hello everyone!

I recently got my, first RNAseq as well as proteomics data set. And thankfully a standard bioinformatic analysis with it. Honestly, I am amazed at what you can find out, when you have the Tools to really dig into your data!

Now, I have been trying (successfully) to replicate the RNAseq data analysis Pipeline by the help of vibecoding with AI, and I can interpret and understand all of my analyses. However, I could never write any bit of Code for it... In the end I don't really understand what the Code does, I can just Review my data afterwards.

Long story short: I would really love to get into bioinformatics a little deeper. Maybe a short term goal would be to build my own fully customizable RNAseq pipeline from scratch and then see from there. Are there any resources you guys with experience can recommend for me?

Thanks!

Dear Maplers,

Yes, yes, yes, Classic World is right around the corner, i know. But I feel like Classic World won't be the ting for me... Anyway, I wanted to get into a PS again and currently most promising two are Fantasia and SlimeTale. I do like the Pasing of ST more, since its 3x from the beginning, however, I do think that removing overused items in Fantasia has a good Catcher as well...

Is there somebody who player both and can give some Feedback? Also do you have Info about the drop Rates in these servers? Are they just 1x or 1x with customizations?

Would love to hear your opinions on these!

Thanks!

Hello Labrats!

I want to do Cell-stretching experiments with C2C12 cells, grown in a PDMS-stretch chamber. Long story short, I need to coat my chambers in order for my cells to adhere. I was thinking of coating with gelatin, as it seems to be the easiest and most cost efficient to try out. However, after coating for either 1h or 4h at 37°C or RT and 4°C overnight, nothing seemed to work and my C2C12 cells start to detach 2 days after seeding. Now, honestly, I question the process of coating cell culture vessels with gelatin as I will incubate my cultures in 37°C and gelatin will liquidify at this temperature? Am I missing something, or can gelatin coating not work in the first place? Do you have any experience with this or any suggestions on how to check for successful coating?
Thanks in advance, and I am happy for any feedback you guys can give me!
Cheers!