u/Interesting-Prior651

Hi everyone,

I'm looking for protocols that are used routinely in diagnostic pathology laboratories for IHC on human FFPE brain and spleen specimens.

Specifically, I'm interested in protocols for:

• T cells (CD4/CD8)
• α-synuclein

If possible, I'd really appreciate details such as:

• Antibody clone/vendor
• Antigen retrieval method (buffer, pH, time)
• Antibody dilution
• Any tissue-specific tips (brain vs spleen)
• Common pitfalls (background staining, fixation issues, etc.)

For context, we're investigating the effects of an infectious disease on the CNS (we are using the spleen as a positive control), and we're hoping to adapt protocols that are already well established in clinical pathology rather than starting from research-only methods.

If your lab stains α-synuclein routinely (e.g., for neurodegenerative pathology) or has a validated CD4/CD8 protocols for FFPE brain/spleen, I'd be very grateful if you could share what works well.

Thanks in advance!

Hi everyone,

I'm looking for protocols that are used routinely in diagnostic pathology laboratories for IHC on human FFPE brain and spleen specimens.

Specifically, I'm interested in protocols for:

• T cells (CD4/CD8)
• α-synuclein

If possible, I'd really appreciate details such as:

• Antibody clone/vendor
• Antigen retrieval method (buffer, pH, time)
• Antibody dilution
• Any tissue-specific tips (brain vs spleen)
• Common pitfalls (background staining, fixation issues, etc.)

For context, we're investigating the effects of an infectious disease on the CNS (we are using the spleen as a positive control), and we're hoping to adapt protocols that are already well established in clinical pathology rather than starting from research-only methods.

If your lab stains α-synuclein routinely (e.g., for neurodegenerative pathology) or has a validated CD4/CD8 protocols for FFPE brain/spleen, I'd be very grateful if you could share what works well.

Thanks in advance!

Hi everyone!

Can anyone recommend a company or organization that sells neuropathology slide study sets (glass slides or digital slide collections) for self-study and board/exam review?

I'm looking for resources that provide a broad range of neuropathology cases and diagnoses for systematic review and teaching purposes. Any recommendations based on your experience would be greatly appreciated.

Thank you!