u/Loud_Fudge2881

Storing mastermix + cDNA overnight for qPCR

Hey guys, I have 12 plates to run over the next couple days and was wondering if it's possible to store my master mix (SYBR, nuc free water, primers) + template overnight ? If not, is it possible to store just the master mix overnight ?

PS: my cDNA is extracted from urinary RNA, so I expect yield to be quite low

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u/Loud_Fudge2881 — 1 day ago

WOW or Asics

Wow AC 13/13E/14, Wow 10/12 or Asics metarise 2/Sky Elite ff 3 ? I already tried : Nike Gt cut 2, my favourite so far, sky elite ff2 were decent but I destroyed them playing outdoor once or twice, Don issue 7 are horrible, too bulky and uncomfortable, traction is meh. I never tried wow, decent experience with asics. For those who tried both, which would you recommend ?

Height: 188 (6"2), Weight: 80 Kgs, outside hitter. I prefer light shoes with good traction and bounce

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u/Loud_Fudge2881 — 3 months ago