Blind spot: trans women
Right off the bat: I am not accusing any of them of transphobia. I am merely pointing out a pattern that i've noticed. Hopefully, once they know better, they will do better.
Mae mentioned a dyke festival they were going to. In trying to explain that the word "dyke" can be inclusive of transmasc folx and trans men, they ended up excluding trans femmes and trans women by saying the festival is for all AFAB (assigned female at birth) people. It was clumsy. I am sure if they had had a bit more time to think through their comments, they would have corrected themselves. But, the way the episode air, it was exclusionary of transfemmes and trans women who identify as dykes (of which there are many).
Similarly, Fortune has joked many many times about being a big lesbian because she has only ever touched one p*nis. This should go without saying but many many women have p*nises. Now, granted, the may choose different names for their private parts that do not have a male connotation. But just making a blank statement that having only touched one p*nis ever is proof of how much of a lesbian you are is pretty problematic.
Edit to add: later on in the episode, Fortune asked Mae if they were drawn to male or female massage therapists. Asking a non-binary pansexual person this is wild.
Once again, I do not think either of them meant to be transphobic. I hope they learn more about this and do better next time.