u/Mo_Opines_968

I'm building a 32 colour panel that is an extension of an existing 27 colour panel that has already been optimised. However I'm going to need to use Fc Block which wasn't needed on the original 27 colour panel.

In the past I've put on Fc block for 10 mins then added antibody mix directly onto cells from that. My concern in this case is that the final staining volume is going to be higher than in the original panel. I'd quite like to avoid having to re-titrate the other 27 antibodies so wondered what people's experience is changing that staining volume e.g. from 50 to 75uL for the same amount of antibody?

Maybe I just need to test it and see...

Thanks!

School closed and am planning to take my kids to We the Curious which I assume is air conditioned.

Would be nice to get them in the fountains after but just wanted to check they were working/it's possible for kids to splash around in them?

Used to go school with Will Taylor and when doing a house clearout found an unopened copy of the EP his band which became Flyte recorded.

Anyone got any interest in this?