u/Ok_Read4513

I got my I-526E denied due to missing supporting documents, but USCIS denied the petition directly without issuing an RFE back in June of this year. I filed an I-290B appeal in July, and I’m wondering if anyone here has gone through the appeal process for an I-526E denial.

What was your timeline like? Has anyone had their I-526E approved during the Initial Field Review before the appeal was transferred to the AAO?

Also, if there is an update, such as approval during the Initial Field Review or transfer to the AAO, would the case status update appear under the I-290B receipt number or the original I-526E receipt number?

I prepared a 10x Genomics GEM-X Universal 3′ Gene Expression v4 4-plex library from dissociated islets. My post-library TapeStation QC shows one main peak at ~316 bp, with an average fragment size of 366 bp across the 150–1000 bp region. Qubit concentration is 4.92 ng/µL.

Has anyone seen a similar final-library profile with this kit? Is a ~316 bp main peak still likely to sequence well or does it suggest over-fragmentation or an issue during double-sided SPRI size selection?

https://preview.redd.it/z170jfzdtlgh1.png?width=1910&format=png&auto=webp&s=0d0a3daa2b55bdabaeede06d28aa66f6a88f55e4