Hello! I'm a trans guy looking to get a haircut soon. I used to go to a friend but they have since moved away.
Anyone have good barbers that are FTM friendly who won't try to give me a pixie cut? I have stick straight hair so no experience with curly hair needed.
I was thinking of just going to sports clips or a barbershop as they always do mens cuts. Any suggestions appreciated!
Hello all! You guys are always super helpful so I thought I'd ask for some assistance here.
TL;DR What is the best way to index/map an assembly so I can easily search for a gene and extract the sequence for comparison to other sequences of the same gene?
I am an amateur and self taught and have been working on some genomics in my free time. I worked with a professor and we wanted to do some whole genome sequencing of two mouse cell lines.
There are some differences in immune responses between these two cell lines so we wanted to see if there were any large scale rearrangements. So far, we haven't identified any large scale rearrangements. I used the Galaxy Project network and created an assembly for each de novo.
Since we didn't find any large scale rearrangements, I was able to use the BUSCO tool on Galaxy to identify some common genes and their location. I then extracted them from the sequence. From there we could compare specific genes at a base pair level. We did actually find some notable differences! My issue is that the BUSCO genes identified are not an exhaustive list and can be a bit clunky to search.
So my question is, what is the best way to index the genes in a whole genome assembly? My goal is to be able to search a list of genes identified and then extract the sequence for that gene for comparison.
Also, I'm aware that making a de novo assembly was maybe not the greatest way to go about things but I'm learning! And I wanted to see what I could do with no reference genome (especially as this specific strain of mouse doesn't have a good reference genome). So if I did something not very smart, please go easy on me!
TL;DR What is the best way to index/map an assembly so I can easily search for a gene and extract the sequence for comparison to other sequences of the same gene?
Hello all! I recently got a new job and am looking to move to the Appleton area. I am hoping to spend $600-700 for my portion of a 2 bedroom place (so ~$1400 total for rent).
I have an ESA that would like to be the only pet running the place! I work 40 hours and am a clean person who doesn't need a ton of space.
I am hoping to move in the next one to two months (Sept or Oct move in date). Would love to hang and see if we vibe before then. If anyone is interested please DM!
Hello! I am looking to load 3 bikes onto my 2019 encore. I am not opposed to adding a hitch to do so, but I was wondering what peoples experience is with hauling bikes with an encore.
Do most people add a hitch so they can attach the rack? Or is there an alternative bike rack type?
Any information is appreciated, thank you!