Can't seem to be able to get SPR to work properly. Helps appreciated.
I have been trying to get an SPR assay (Biacore t200) to work between a protein ~32 kDa and ATP, but it is going nowhere despite spending so much on chips (I am afraid my PI will just stop me from trying it anymore). I use CM5 amine coupling to immobilize the protein and flow buffer containing various concentrations of ATP through. This image is literally the first time I see one concentration (3.7) that appears to have worked. All the runs have been looking like the other concentrations here, which seem like no binding at all. This kinda gives me some hope, but it has been so depressing trying so many different things that nothing really works. I desperately need this assay to work for my first paper 😞. Any helps/sugegstions are greatly appreciated.