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u/Ultronomy

r/ask r/labrats r/AskALiberal r/Flooring
▲ 0 r/AskALiberal

Do you believe “False Flags” are occurring in the US regularly?

Every time an extremist group on either side of the political aisle does something, people left and right claim “false flag” or “agents!” This can be seen with Patriot Front and Antifa pretty regularly. To me this kind of just demonstrates the divide in our country that we can’t fathom there actually being these extremist groups. I personally do believe extremists exist on both sides and don’t necessarily believe the government is sewing division in this way. I do fully believe the current admin is sewing division though.

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u/Ultronomy — 5 hours ago
▲ 4 r/ask

Is censorship on media sites like YouTube beneficial?

I am an avid YouTube watcher, and over the past few years I’ve noticed a stark increase in the censorship of more and more words. This includes words like: pill, drugs, child, etc. I’ve heard they do it because advertisers effectively require it. However, this just makes me wonder, is a significant portion of the population genuinely offended/hurt by such words? Is there a demonstrative decline in profits by allowing these words? I’ve always been anti-censorship, and it is getting to a point where I am genuinely annoyed by it.

reddit.com
u/Ultronomy — 9 days ago
▲ 170 r/Flooring

First time homeowner and DIYer. It ain’t pretty but the floors and baseboards are done.

u/Ultronomy — 1 month ago
▲ 3 r/labrats

Help with determining confluence?

I know I should just count the cells using a hemocytometer… I am still waiting for them to arrive. I am merely looking for someone to fact check my estimates.

Picture 1: 40%
Picture 2: <30%
Picture 3: 50-60%
Picture 4: 40%

I’ve posted here before about cells. As I’ve mentioned in previous posts, I am the primary person working with mammalian cells (RAW264.7) in my Chem Bio research group. And by extension, the only person on our campus working with an immortalized cell line. So, I am by no means an expert and don’t have a ton of people to ask for help from.

u/Ultronomy — 1 month ago
▲ 1 r/labrats

Hello fellow lab rats! I am a Chemical Biologist doing 70% synthesis and 30% cell culture with the materials I synthesize. I am unfortunately, considered the cell expert in my group. Alas, I don’t feel like am expert despite doing this for 4 years. I have been struggling with my RAW cells obtained from ATCC. The first batch I got looked dead on arrival and showed no growth after over a week. This batch, looks better but is growing a lot slower than I am used to. Overall, they have grown substantially since I last check on Saturday, but since Monday they have seemed stagnant.

I am very confident my freshly made DMEM with 10% FBS isn’t contaminated and I used a fresh bag of T75s. For thawing, I thawed in roughly 2 minutes in bead bath and diluted the cryo-media to 10 mL with DMEM/FBS before centrifuging. Didn’t blow a bunch of bubbles through them while re-suspending… all of this was of course done in a bio-hood. I am very confident my procedures have all been kosher.

This is my third time thawing a fresh batch from ATCC. The first time the cells were ready to passage after two days. And this is typically what I experience with my own frozen stocks (of which I have no more currently). Do I just wait it out and keep maintaining them? They are certainly not ready to passage yet. I am hoping it’s just mild stress from the thawing process they are trying to recover from?

u/Ultronomy — 2 months ago
▲ 1 r/Flooring

I know the usual motto is to just lay the LVP right over it… but I have to scrape off the remaining foam from the carpets to do that (and it doesn’t come off nicely). Is it much better to be on my hands and knees scraping foam off this crumbly tile? Or at that point should I just get it removed? I’ll still wear a mask, just asking.

It’s also going to mean the bedroom and living room will be unleveled if I keep it.

u/Ultronomy — 2 months ago