u/anto3003

Image 1 — Help with SW-620 cell line?
Image 2 — Help with SW-620 cell line?
Image 3 — Help with SW-620 cell line?

Help with SW-620 cell line?

Hello everybody, sorry for the english but it's not my first language. I just started working with some SW-620 colorectal cancer cell lines that have been in nitrogen for a few years. Nobody in my lab has worked with them before (the guy who did left a while ago) so i decided to reach out here. I put a picture of what i've seen them look like in litterature vs what i see in the microscope. As you can see, our cells don't present that morphology, they don't seem to go past that "rounded" state (i'm so sorry if it is complicated to understand or if i use the wrong words).
Anyways, do you know something about this cell line in culture? Does it take a while to see that morphology or is this normal?
Thank you in advance for any answers or advice you could give me and i hope you have a nice day! :)

u/anto3003 — 5 days ago

Looking 4 someone to analyze data

Hello everybody. I'm in a huge time crunch with deadlines and i have absolutely no time to analyze some data from one of my students . I'm looking for someone who can help me with it (💸 ofc). It's a full cell cycle analysis using 7-aad and ki-67 coupled to AF-488. There are 4 cell lines, 4 timepoints and each one with 2 conditions (so basically 32 samples w/o counting the compensation controls). I took a peak at the data and it was ok-ish. If anybody is interested please message me. Thanks and have a good day!

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u/anto3003 — 2 months ago