r/cellculture

Hello everybody, sorry for the english but it's not my first language. I just started working with some SW-620 colorectal cancer cell lines that have been in nitrogen for a few years. Nobody in my lab has worked with them before (the guy who did left a while ago) so i decided to reach out here. I put a picture of what i've seen them look like in litterature vs what i see in the microscope. As you can see, our cells don't present that morphology, they don't seem to go past that "rounded" state (i'm so sorry if it is complicated to understand or if i use the wrong words).
Anyways, do you know something about this cell line in culture? Does it take a while to see that morphology or is this normal?
Thank you in advance for any answers or advice you could give me and i hope you have a nice day! :)

Hey guys! I’ve attached a picture with all the information about the app.

In short:

I’m a PhD student, and I originally made this app for myself. I then thought it might be useful for others, so I decided to publish it on the AppStore.

There’s absolutely no commercial motive. The app is completely free, and I just want to help my colleagues.

The biggest problem is: it’s extremely difficult to get the app in front of people. Most communities don’t allow app promotion, and obviously I can’t afford to pay for advertising.

So, I’d really appreciate it if you could share it with other people.

I’m open to all kinds of feedback, positive or negative, so please be honest.

If there’s a feature you’d like to see, let me know. I may be able to add it in the next few days.

Thanks a lot!

I am experimenting with 3T3 and got contamination and cells are growing well, and the media color remains the same but these particles I think are contamination. Can you help with these?

As the title says, we have two moldy incubators that no matter how many deep cleans we do, the mold comes back.

I've been in this lab for a year for my PhD and it's giving me anxiety. The PI is very nonchalant about him and thinks it's "normal" and says that would always happen once in a while when he was doing manips back in his day. Everyone else in the lab has been here for longer than I amd some of them have never done another lab so they've also become a bit indifferent about it.

I literally lose sleep over it. I have very long cultures that require minimal media exchange per the protocol so I have a pretty big risk that mold might eventually develop in my cultures compared to my labmates who change their media twice a week.

From what I've been told, it's always the same kind of mold that comes back (per the pictures). They do deep cleans by following a specific protocol that involves autoclave every part of the incubator + 3M soap but there's no use. Last deep clean was less than 3 months ago and it's already this bad.

For reference, we have 5 incubators of the same model and 2 of them have the mold issue and both are in the same culture room (though I've never seen the second one in that room turned one. It's just there, door closed and turn off). The other 3 are in two different rooms. This model does not have filters for the air or the CO2, but it has a UV light around the waterbath. It does not have an autoclean mode.

I was hoping anyone might have a suggestion? Anything really

Thanks