




Mold incubator despite regular cleanings
As the title says, we have two moldy incubators that no matter how many deep cleans we do, the mold comes back.
I've been in this lab for a year for my PhD and it's giving me anxiety. The PI is very nonchalant about him and thinks it's "normal" and says that would always happen once in a while when he was doing manips back in his day. Everyone else in the lab has been here for longer than I amd some of them have never done another lab so they've also become a bit indifferent about it.
I literally lose sleep over it. I have very long cultures that require minimal media exchange per the protocol so I have a pretty big risk that mold might eventually develop in my cultures compared to my labmates who change their media twice a week.
From what I've been told, it's always the same kind of mold that comes back (per the pictures). They do deep cleans by following a specific protocol that involves autoclave every part of the incubator + 3M soap but there's no use. Last deep clean was less than 3 months ago and it's already this bad.
For reference, we have 5 incubators of the same model and 2 of them have the mold issue and both are in the same culture room (though I've never seen the second one in that room turned one. It's just there, door closed and turn off). The other 3 are in two different rooms. This model does not have filters for the air or the CO2, but it has a UV light around the waterbath. It does not have an autoclean mode.
I was hoping anyone might have a suggestion? Anything really
Thanks