Issue dissolving catalase
Hi everyone! I have a bit of an annoying issue in the lab and I'm hoping someone might be able to share some insight that helps
I purchased 50 mg of catalase enzyme and need to prepare a stock solution of 0.8 mg/mL - the purpose is to use it in an imaging buffer for single molecule localisation microscopy, catalase is used alongside glucose oxidase to reduce oxidative photobleaching.
This afternoon, I tried to dissolve 12 mg in 15 mL of my buffer (10 mM HEPES, 150 mM NaCl, 10 mM MgCl2, pH 7.4) but found it didnt dissolve very well at all. I tried heating at 30°C for an hour or so but that didnt seem to help. I then tried centrifuging, discarding the buffer and attempting to dissolve in distilled water, to no success.
The internet tells me that it should dissolve easily up to 1 mg/mL in water and neutral buffers (specifically mentioning phosphate buffer, which I will try next if nothing else)
Does anyone have any experience or advice? Thanks in advance!