u/faux_adult

[University: Mass spectrometry] Calculating mass from spectrum

[University: Mass spectrometry] Calculating mass from spectrum

The exercicr doesn't say what type of spectrometry ot is but from the context I assume it's MALDI-TOF. The other kind of mass spectrometry we've seen in class is Electrospray-TOF.

So I'm suposed to calculate the mass of a protein using info from peaks A and B, or any other pair of peaks. Then calculate the charge of peak C. If it's MALDI-TOF I would assume peak B is the single-protonated protein, but if I try to do an equation system with other peaks it doesn't hold up. If it were Electrospray-TOF I think the m/z would change in regular intervals?

Help, my teacher thinks this is too easy to properly explain.

u/faux_adult — 1 day ago