![[University: Mass spectrometry] Calculating mass from spectrum](https://preview.redd.it/6sglnebqb8bh1.png?auto=webp&s=413423b7f191e21db3e34e78e1ea77ecce41ae26)
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[University: Mass spectrometry] Calculating mass from spectrum
The exercicr doesn't say what type of spectrometry ot is but from the context I assume it's MALDI-TOF. The other kind of mass spectrometry we've seen in class is Electrospray-TOF.
So I'm suposed to calculate the mass of a protein using info from peaks A and B, or any other pair of peaks. Then calculate the charge of peak C. If it's MALDI-TOF I would assume peak B is the single-protonated protein, but if I try to do an equation system with other peaks it doesn't hold up. If it were Electrospray-TOF I think the m/z would change in regular intervals?
Help, my teacher thinks this is too easy to properly explain.
u/faux_adult — 1 day ago